Presentations & Publications

Senti Bio’s team is pioneering the use of Gene Circuits. The publications highlighted below represent a sample of the team’s innovative and foundational work.

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August 2024

Precision off-the-shelf natural killer cell therapies for oncology with logic-gated gene circuits

Cell Reports
Nicholas W. Frankel, Han Deng, Gozde Yucel, Marcus Gainer, Nelia Leemans, Alice Lam, Yongshuai Li, Michelle Hung, Derrick Lee, Chen-Ting Lee, Andrew Banicki, Mengxi Tian, Niran Almudhfar, Lawrence Naitmazi, Assen Roguev, Seunghee Lee, Wilson Wong, Russell Gordley, Timothy K. Lu, and Brian S. Garrison

May 2024

Iterative Engineering of Polarization-State Responsive Synthetic Promoters for Autonomous Control of Macrophage Polarization Logic

ASGCT Annual Meeting
Frances Liu, Michelle Hung, Assen Roguev, Magdalena Cichewicz, Yin Yin Chong, Cesar Juarez, William Hendriks, Jessica Haverkamp, Mark Tomishima, Timothy Lu, Russell Gordley, and Philip Lee

May 2024

Engineered IMiD Regulated Synthetic Transcriptional Switch for Controlled and Dose-Responsive Expression of Therapeutic Payload within FDA-Approved Drug Doses

ASGCT Annual Meeting
Rebecca Cottman, Myles MacEachern, Michelle Hung, Andrew Banicki, Mengxi Tian, Chen-Ting Lee, Assen Roguev, Chew-Li Soh, Conor McAuliffe, Mark Tomishima, Raghav Kannan, Chandra Verma, Timothy K. Lu, Russell Gordley, Philip Lee

May 2024

Discovery of Novel CARs for Solid Tumors Using Senti REVEAL™

ASGCT Annual Meeting
Nick Frankel, PhD

May 2024

Precision off-the-shelf natural killer cell therapies for oncology with logic-gated gene circuits

Cell Reports
Nicholas W. Frankel, Han Deng, Gozde Yucel, Marcus Gainer, Nelia Leemans, Alice Lam, Yongshuai Li, Michelle Hung, Derrick Lee, Chen-Ting Lee, Andrew Banicki, Mengxi Tian, Niran Almudhfar, Lawrence Naitmazi, Assen Roguev, Seunghee Lee, Wilson Wong, Russell Gordley, Timothy K. Lu, Brian S. Garrison

April 2024

Preclinical data supporting the Phase 1 trial design of SENTI-202, a next generation allogeneic logic-gated selective CAR-NK cell therapy, engineered to overcome key limitations of first generation cell therapies in AML

AACR Annual Meeting
Deepika Kaveri, Pearley Chinta, Elizabeth Leitner, Lawrence Naitmazi, Gozde Yucel, Mengxi Tian, Niran Almudhfar, Han Deng, Yongshuai Li, Alice Lam, Chen-Ting Lee, Enping Hong, Rochelle Emery, Brian Garrison, Aaron N. Nguyen, and Kanya Rajangam

December 2023

Calibrated Release IL15-Expressing Bivalent CD33 and/or FLT3 Logic Gated Gene Circuit CAR-NK Cell Therapy (from SENTI-202 gene circuit) in Venetoclax Resistant Patient Derived Xenograft Acute Myeloid Leukemia Models

ASH Annual Meeting
Muharrem Muftuoglu, Mahesh Basyal, Li Li Jiangxing, Lauren B. Ostermann, Ran Zhao, Baozhen Ke, Hiroki Akiyama, Po Yee Mak, Bing Z. Carter, Han Deng, Yongshuai Li, Brian S. Garrison, Kanya L. Rajangam, Michael Andreeff

October 2023

Smart sensor promoters drive state-specific gene circuits to convert immunosuppressive macrophages into an anti-tumor phenotype

SITC Annual Meeting
Frances D. Liu, Michelle Hung, Assen Roguev, Yin Yin Chong, Cesar Juarez, Russell M. Gordley, Mark Tomishima, Jessica M Haverkamp, Panos Douvaras, Alex Danza, Monika Avina, Timothy K. Lu, and Philip Lee

October 2023

Small-molecule regulated safety switch for improved safety of CAR cell therapies in the brain

SITC Annual Meeting
Rebecca T. Cottman, Vanessa Johnson, Michelle Hung, Yin Yin Chong, Eren Chu, Assen Roguev, Marcus Gainer, Joseph Sperling, Monika Avina, Brett Kiedaisch, Raghav Kannan, Chandra Verma, Chew-Li Soh, Conor McAuliffe, Mark Tomishima, Russell M. Gordley, Timothy K. Lu, and Philip Lee

October 2023

NOT gate gene circuits expand the range of tumor-associated antigens addressable by CAR-NK and -T cell therapies

SITC Annual Meeting
Nicholas W. Frankel, Assen Roguev, Marcus Gainer, Han Deng, Priscilla Wong, Gozde Yucel, Alice Lam, Yongshuai Li, Michelle Hung, Derrick Lee, Chen-Ting Lee, Andrew Banicki, Mengxi Tian, Niran Almudhfar, Wenqi Song, Lawrence Naitmazi, Brian Garrison, Kanya Rajangam, Timothy K. Lu

October 2023

High-throughput discovery of constitutive promoters to drive strong expression of next-generation gene circuits in CAR-NK and -T cells

SITC Annual Meeting
Tony Hua, Nicholas W. Frankel, Ippolito I. Caradonna, Assen Roguev, Karen L. Chu, Russell M. Gordley, Kanya Rajangam and Timothy K. Lu

May 2023

Massively Parallel and Systematic Engineering Platform for Highly Compact, Cell-type Specific, and Potent Smart Sensor Promoters for Precision Retinal Gene Therapies

ASGCT Annual Meeting
Joseph Draut, Thant Zaw, Myles MacEachern, Magdalena Cichewicz, Assen Roguev, Jang Hwan Cho, Daniel M. Cohen, Laura Barrio Real, Shreyasi Choudhury, Ali Nahvi, Jed Chatterton, Virginia A. Haurigot, Sean M. Armour, Federico Mingozzi, Rocky Chueng, Michelle Hung, Frances Liu, Rebecca Cottman, Nicholas Frankel, Tony Hua, Gary K. Lee, Curt Herberts, Philip Lee, Timothy Lu, Russell Gordley

May 2023

Engineering a Gene Circuit-Enabled Cell Therapy with a Tamoxifen Regulated Safety Switch for Inducible Cell Death in Human Pluripotent Stem Cells and their Derivatives

ASGCT Annual Meeting
Chew-Li Soh, Rebecca Cottman, Conor McAuliffe, Vanessa Johnson, Michelle Hung, Yin Yin Chong, Noah Jacobs-Rebhun, Cloe Grace, Jeffrey Levin, Tackla Winston, Mark Ebel, Eren Chu, Assen Roguev, Russell Gordley, Tim Lu, Mark Tomishima

May 2023

High-throughput engineering of Logic Gated-gene circuits for precision CAR cell therapies

ASGCT Annual Meeting
Nicholas W. Frankel, Ippolito I. Caradonna, Marcus A. Gainer, Junjie Tony Hua, Brian Garrison, Russell M. Gordley, Timothy K. Lu

May 2023

Designing cell-state-specific synthetic promoters as Smart Sensors to control macrophage polarization

ASGCT Annual Meeting
Frances D. Liu, Michelle Hung, Assen Roguev, Yin Yin Chong, Eniola Catton, Jessica Haverkamp, Panos Douvaras, Alex Danza, Monika Avina, Mark Tomishima, Russell Gordley, Gary K. Lee, Timothy Lu

May 2023

Engineering pharmacologically relevant, FDA-approved small-molecule-regulated gene circuits for therapeutic applications in the brain

ASGCT Annual Meeting
Rebecca Cottman, Vanessa Johnson, Michelle Hung, Yin Yin Chong, Andrew Banicki, Mengxi Tian, Chen -Ting Lee, Eren Chu, Assen Roguev, Monika Avina, Alex Danza, Brett Kiedaisch, Raghav Kannan, Chandra Verma, Chew -Li Soh, Conor McAuliffe, Mark Tomishima, Russell Gordley, Gary Lee, Tim Lu

April 2023

Off-the-Shelf CAR-NK Cells Engineered to Express Calibrated Release IL-15 Exhibit Enhanced Persistence and Anti-Tumor Activity

AACR Annual Meeting
Kelly Chen-Ting Lee, Michelle Hung, Andrew Banicki, Wenqi Song, Niran Almudhfar, Deepika Kaveri, Priscilla Wong, Lawrence Naitmazi, Marcela Guzman, Alice Lam, Gozde Yucel, Timothy Lu, Alba Gonzalez Junca, Brian Garrison and Philip Lee

April 2023

Preclinical development of SENTI-202, an off-the-shelf Logic Gated CAR-NK cell therapy, for the treatment of CD33/FLT3+ hematologic malignancies including AML

AACR Annual Meeting
Alba Gonzalez, Enping Hong, Gozde Yucel, Elizabeth Leitner, Pearley Chinta, Han Deng, Ian Li, Alice Lam, Abla Bakir, Brandon Lee, Papia Chakraborty, Carmina Blanco, Chen-Ting Lee, Niran Almudhfar, Mengxi Tian, Wenqi Song, Andrew Banicki, Otto Contreras, Martin Gieldin, Brian Garrison, Timothy K. Lu, Kanya Rajangam

April 2023

SENTI-301A, an Off-the-Shelf Multi-Armed Preclinical CAR-NK Cell Therapy for the Treatment of GPC3 Expressing Tumors

AACR Annual Meeting
Deepika Kaveri, Enping Hong, Elizabeth Leitner, Priscilla Wong, Ronni Ponek, Lawrence Naitmazi, Pearley Chinta, Wesley Gorman, Mengxi Tian, Niran Almudhfar, Kelly Lee, Nicholas Frankel, Russell Gordley, Philip Lee, Alba Gonzalez Junca, Timothy Lu, Kanya Rajangam, Marcela Guzman Ayala

December 2022

SENTI-202, a Selective, Off-the-Shelf, Preclinical CAR-NK Cell Therapy with CD33 and/or FLT3 Activating CAR, Healthy Cell Protection from Endomucin (EMCN) Inhibitory CAR and Calibrated Release IL-15 for Hematologic Malignancies Including AML

ASH Annual Meeting
Brian Garrison, Han Deng, Gozde Yucel, Nicholas Frankel, Russell Gordley, Michelle Hung, Derrick Lee, Marcus Gainer, Assen Roguev, Wesley Gorman, Nelia Leemans, Alice Lam, Yongshuai Li, Wilson Wong, Kanya Rajangam, Philip Lee, Timothy Lu

November 2022

Selection of Lead Development Off-the-shelf CAR NK Candidate, SENTI-301A, for GPC3 Program and Expansion of Regulator Dial Gene Circuits to Tackle Key Challenges In Solid Tumors

SITC Annual Meeting
Marcela Guzman Ayala, Michelle Hung, Deepika Kaveri, Rebecca Cottman, Enping Hong, Elizabeth Leitner, Priscilla Wong, Ronni Ponek, Lawrence Naitmazi, Manan Shah, Wesley Gorman, Andrew Banicki, Mengxi Tian, Niran Almudhfar, Kelly Lee, Russell Gordley, Philip Lee, Timothy Lu

November 2022

SENTI-401, an Allogeneic Logic-Gated and Multi-Armed CAR-NK Cell Therapy for the Treatment of CEA-Expressing Solid Tumors with Enhanced Selectivity and Activity

SITC Annual Meeting
Nicholas Frankel, Maelig Morvan, Assen Roguev, Michelle Hung, Russell Gordley, Miguel Palermo, Tyler Santomasso, Pearley Chinta,Aldo Sotelo, Marcus Gainer, Derrick Lee, Tony Hua, Cheng-Ting Lee, Andrew Banicki, Mengxi Tian, Niran Almudhfar, Otto Contreras, Tim Lu, Alba Gonzalez

May 2022

Logic Gated FLT3 OR CD33 NOT EMCN CAR-NK Cell Therapy (SENTI-202) for Precise Targeting of AML

ASGCT Annual Meeting
Brian Garrison , Han Deng, Gozde Yucel, Nicholas Frankel, Russell Gordley, Michelle Hung, Derrick Lee, Marcus Gainer, Nelia Leemans, Alice Lam, Wilson Wong, Philip Lee, Tim Lu, Gary Lee

May 2022

Multi-Arming and Regulator Dial Gene Circuits to Address Key Disease Challenges in HCC

ASGCT Annual Meeting
Marcela Guzman Ayala, Michelle Hung, Poornima Ramkumar, Deepika Kaveri, Elizabeth Leiner, Priscilla Wong, Ronni Ponek, Brett Kiedaisch, Wesley Gorman, Russell Gordley, Rebecca Cottman, Kelly Lee, Allison Drain, Ahmad S Khalil, Gary Lee

May 2022

Activation regulated gene circuit for controlling payload expression in cell therapies

ASGCT Annual Meeting
Michelle Hung, Assen Roguev, Yin Yin Chong, Carmina Blanco, Travis Wood, Brandon Lee, Brett Kiedaisch, Russell Gordley, Gary Lee, Tim Lu

May 2022

Process Development and Scale-Up for Gene Circuit Engineered CAR-NK Cell Manufacturing

ISCT Annual Meeting
Travis Wood, Abla Bakir, Carmina Blanco, Dharini Iyer, Wesley Gorman, Haritha Lakshmireddy, Charity Vilchez Juang, Denny Nguyen, Martin Giedlin, Philip Lee

April 2022

Driving anti-tumor activity in solid tumors with controlled arming of allogeneic CAR-NK

AACR Annual Meeting
Alba Gonzalez, Michelle Hung, Marcela Guzman, Aldo Sotelo, Nicholas Frankel, Yin-Yin Chong, Deepika Kaveri, Poornima Ramkumar, Elizabeth Leiner, Priscilla Wong, Ronni Ponek, Kelly Lee, Alyssa Mullenix, Russell Gordley, Gary Lee

December 2021

FLT3 OR CD33 NOT EMCN Logic Gated CAR NK Cell Therapy (SENTI 202) for Precise Targeting of AML

ASH Annual Meeting
Brian S. Garrison, PhD , Han Deng, PhD, Gozde Yucel , PhD, Nicholas W. Frankel, PhD, Marcela Guzman Ayala, PhD, Russell Gordley, PhD, Michelle Hung, PhD, Derrick Leem, Marcus Gainer, Kathryn Loving, PhD, Jenny Chien, Tiffany Pan, MS, Wesley Gorman, Nelia Leemans , MS, Alice Lam, Poornima Ramkumar, PhD, Travis Wood, Wilson Wong, PhD, Philip Lee, PhD, Tim Lu, MD PhD, Gary Lee, PhD

November 2021

Development of Logic Gated CAR-NK cells for the Treatment of Solid Tumors

SITC Annual Meeting
Alba Gonzalez, Assen Roguev, Brian Garrison, Nicholas Frankel, Derrick Lee, Marcus Gainer, Alyssa Mullenix, Russel Gordley, Kathryn Loving, Jenny Chien, Gary Lee

May 2021

Umbilical Cord Blood derived Natural Killer Cells Provide a Highly Scalable Source For Gene Circuit-Engineered Allogeneic CAR-NK Therapies

ISCT Annual Meeting
Dharini Iyer, Wesley Gorman, Carmina Blanco, Travis Wood, Mario Lorente, Charity Vilchez Juang, Denny Nguyen, Brandon Lee, Brett Kiedaisch, Philip Lee

May 2021

Scalable GMP-Ready Manufacturing Process for Gene Circuit Engineered Allogeneic CAR-NK Cell Therapy for Cancer

ISCT Annual Meeting
Travis Wood, Abla Bakir, Carmina Blanco, Dharini Iyer, Brett Kiedaisch, Wesley Gorman, Mario Lorente, Brandon Lee, Denny Nguyen, Philip Lee

May 2021

Small-molecule-regulated Gene Circuit for Controlling Cytokine Expression in Cell Therapies

ASGCT Annual Meeting
Michelle Hung, Divya Israni, Huishan Li, Yin Yin Chong, Poornima Ramkumar , Allison Drain, Allison Quach, Mengxi Tian, Rishi Savur, Niran Almudhfar, Chen Ting Lee, Abla Bakir, Edwin Cruz, Carmina Blanco, Travis Wood, Mario Lorente, Brandon Lee, Brett Kiedaisch, Russell M. Gordley, Marcela Guzman Ayala, Ahmad Khalil, Gary Lee, Tim Lu

May 2021

Precise Targeting of AML With First-in-Class OR/NOT Logic-Gated Gene Circuits in CAR-NK Cells

ASGCT Annual Meeting
Brian S. Garrison, Han Deng, Gozde Yucel , Nicholas W. Frankel, Marcela Ayala Guzman, Russell M. Gordley , Michelle Hung, Derrick Lee, Marcus Gainer, Kathryn Loving, Jenny Chien , Tiffany Pan, Wesley Gorman, Nelia Leemans , Alice Lam, Travis Wood, Wilson Wong, Philip Lee, Tim Lu, Gary Lee

May 2021

Precise Tumor Targeting with NOT GATE Chimeric Antigen Receptor Gene Circuits

ASGCT Annual Meeting
Nicholas W. Frankel, Seunghee Lee, Derrick Lee, Marcus Gainer, Brian S. Garrison, Travis Wood,Wesley Gorman, Russell Gordley, Marcela Guzman Ayala, Tim Lu, Gary Lee, Wison Wong

April 2021

Development of logic-gated CAR-NK cells to reduce target-mediated healthy tissue toxicities

AACR Annual Meeting
Alba Gonzalez-Junca, Assen Roguev, Brian Garrison, Nicholas Frankel, Derrick Lee, Marcus Gainer, Alyssa Mullenix, Russell Gordley, Kathryn Loving, Jenny Chien, Gary Lee

April 2018

Universal chimeric antigen receptors for multiplexed and logical control of T cell responses

Cell
Cho JH, Collins JJ, Wong WW. Universal Chimeric Antigen Receptors for Multiplexed and Logical Control of T Cell Responses. Cell 173:1426 (2018). doi: 10.1016/j.cell.2018.03.038.

April 2018

Generalized extracellular molecule sensor platform for programming cellular behavior

Nature Chemical Biology
Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M. Nature Chemical Biology 14:723 (2018).  doi: 10.1038/s41589-018-0046-z.

October 2017

Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy

Cell
Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki H, Stupp D, Wehrspaun C, Tabach Y, Sharp PA, Lu TK. Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy. Cell 171:1138 (2017). doi: 10.1016/j.cell.2017.09.049.

April 2016

A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells

Nature Communications
Saxena P, Heng BC, Bai P, Folcher M, Zulewski H, Fussenegger M. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells. Nature Communications 7: 11247 (2016). doi: 10.1038/ncomms11247.

December 2015

Implantable synthetic cytokine converter cells with AND-gate logic treat experimental psoriasis

Science Translational Medicine
Schukur L, Geering B, Charpin-El Hamri G, Fussenegger M. Implantable synthetic cytokine converter cells with AND-gate logic treat experimental psoriasis. Science Translational Medicine 7:318 (2015). doi: 10.1126/scitranslmed.aac4964.